STABLE ISOTOPE LABELED PRECURSORS FOR NMR APPLICATIONS

About us



Commissariat à l'Energie Atomique et aux énergies alternatives (CEA)

CEA is a French government-funded technological research organisation established in 1945. A leader in research, development and innovation, the CEA is mainly active in 4 domains: low-carbon energies, defense and security, information technologies and health technologies.
The CEA-Grenoble is located in the heart of a rich scientific, industrial and academic environment and groups together fundamental research and technological innovations serving industry.

The NMR-Bio unit is born from collaboration between two CEA/CNRS/UJF laboratories, the Biomolecular NMR Spectroscopy Group of the Structural Biology Institute (IBS) and the Laboratory of Chemistry and Biology of Metals of the Research Institute of Science and Technologies for Living (iRTSV), both located in Grenoble, France.

NMR-Bio is hosted by the iRTSV & IBS and, with the CEA support, distributes patented labelled precursors for the production of methyl-specific labelled proteins of interest with stable isotopes. Since 2014, NMR-BIO is offering customized NMR services ranging from the production of labelled proteins to the acquisition and analysis of high field NMR data.


Biomolecular NMR Spectroscopy Group of Institut de Biologie Structurale (CNRS/CEA/UJF) (Biomolecular NMR Group)

IBS Biomolecular NMR group is developing innovative NMR spectroscopy techniques and new isotopic labelling approaches to address current and future challenges in structural biology. Studies such as large RNA/protein complexes, protein folding/unfolding, self-assembling, as well as mechanisms of large molecular machines are targeted.
Lately, the group has expanded in order to exploit the power of solid-state NMR to study the structure and dynamics of membrane proteins and large assemblies.

Recently in this group, the team of Dr. J. Boisbouvier has developed in collaboration with LCBM, new isotopic labelled precursors that allow the labelling of any combination of methyl groups in a fully perdeuterated proteins without any detectable scrambling. These new precursors are particularly important to increase the number of useful methyl probes available to investigate the detail structure, dynamics and mechanism of large biomolecular assemblies by NMR.


Laboratory of Chemistry and Biology of Metals (CNRS/CEA iRTSV/UJF) (Laboratoire de Chimie et Biologie des Metaux) The LCBM has a large expertise in biology, protein chemistry, synthetic chemistry, development of bioinspired catalysts and physical chemistry and develops research in order to provide new insights into the unique question of the physiological function, structure, chemical reactivity and regulation of complex biological systems transporting and utilizing metal ions. During the last decade this laboratory has developed homogeneous and heterogeneous metal-based (photo)catalysts for the oxidation of organic substrates.
In collaboration with Biomolecular NMR group of IBS, the team of Dr O. Hamelin is developing synthetic schemes to produce stable-isotope-labelled metabolic precursors for the specific labelling of methyl groups of amino acids in proteins.


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