How we work: efficient NMR spectroscopy methods for high-precision research.

At NMR-Bio, we are dedicated to providing world-class Nuclear Magnetic Resonance (NMR) spectroscopy services through privileged access to the state-of-the-art NMR platform of IBS. Leveraging high-field NMR instruments and advanced NMR techniques, we support protein R&D and biopharmaceutical research with precise, high-resolution data. As a Contract Research Organization (CRO), our goal is to deliver the structural information and insights that drive innovation and accelerate drug development.

We collaborate closely with our clients, offering tailored NMR experiments that address specific research objectives – whether it’s elucidating small molecule – protein interactions or tackling more complex biomolecular challenges in drug discovery and biologics R&D. By combining scientific expertise with cutting-edge NMR technology, we ensure efficiency, accuracy, and swift results. Our collaborative approach empowers researchers to make data-driven decisions with confidence, shaping the future of life science research.

A structured process for high-precision NMR research.

At NMR-Bio, we follow a rigorous and structured process to ensure precision, efficiency, and reliability at every stage of your project. Here are the 5 key steps that define our workflow :

1. Initial Meeting
and Agreement

We begin with a thorough discussion to understand the client’s requirements, objectives, and research challenges. This step includes:

Signing a Non-Disclosure Agreement (NDA) to ensure confidentiality.
Reviewing relevant protocols and aligning on research goals.
Establishing clear parameters for NMR research and development using NMR spectroscopy and other analytical methods.

2. Feasibility Study

A comprehensive feasibility study is performed to assess the viability of the project. This phase may include:

Design of several constructs (if needed), expression optimization and development of labeling protocols.
Purification optimization and Quantification of production yields

Sample quality control using advanced NMR technology and mass spectrometry (MS).

3. Large-Scale
Production

Upon validation of the feasibility study, we proceed with large-scale production tailored to meet the client’s specific needs. This phase includes:

Production of the desired protein or biomolecule, with isotopic labeling optimized for NMR spectroscopy.
Consideration of factors such as protein size, dynamics, expression system, and structural elucidation to ensure compatibility with NMR.

4. NMR Data Acquisition
and Analysis

Using state-of-the-art NMR spectrometers and high-field instrumentation, we perform precise data acquisition and analysis, including:

High-resolution NMR spectra collection for structural determination and molecular elucidation.
Analysis of biological systems, including complex protein machineries , enzymes, antibodies or peptides.
Sample quality assessment, ligand interaction screening, identification of interaction sites, conformational changes, and structural dynamics,
Orthogonal techniques such as ITC (Isothermal Titration Calorimetry) and SPR (Surface Plasmon Resonance) are used as complementary methods when required.

5. Technical Report

A comprehensive technical report is provided to the client, summarizing:

Collected NMR data and findings.
Recommendations for further steps based on structural elucidation, spectral analysis, and biological research insights.
Technical service support and guidance for ongoing or future research.

We combine advanced NMR technology with a meticulous, step-by-step approach to ensure precision and reproducibility throughout your research project. Our process is designed to deliver high-quality results tailored to your unique research objectives.

Advanced NMR kits for isotopic labeling: optimized solutions for research and development.

In addition to our services, NMR-Bio offers ready-to-use kits specifically designed for the isotopic labeling of methyl groups along with deuteration in recombinant proteins produced in bacterial expression systems.

These kits simplify the labeling process, providing researchers with high-quality, isotopically-labeled proteins optimized for NMR studies.

By streamlining the labeling process, our kits enhance the quality and reliability of NMR data, facilitating studies on high molecular weight proteins and enabling accurate insights for complex research projects.

Our catalog includes a range of isotopic labeled proteins tailored to meet the specific requirements of NMR and MS analyses.

These solutions enable efficient and precise structural characterization or absolute quantification, supporting research efforts in structural biology, molecular analysis, and other related fields.

For more information, check out our article on the advantages of methyl isotope labeling for studying high molecular weight proteins.