Labeled amino acids and precursors for advanced protein research.
At NMR-Bio, we manufacture and distribute a range of high-quality isotope-labeled amino acids and precursors designed to enhance protein enrichment in stable isotopes. Through these products, optimized protein labeling schemes are achieved which are essential for researchers working on NMR atomic characterization of biological objects and chemical molecules.
Our labeled amino acids, such as L-alanine, L-methionine, L-threonine, and BCAA, support advanced NMR studies, allowing for deeper insights into protein structure, dynamics, and cellular functions. To explore how isotope-labeled compounds can advance your research, check out the historical evolution of isotope enrichment in proteins for NMR applications.
Methyl labeling: advancing protein characterization with isotopically labeled amino acids for NMR studies.
Methyl-specific isotope-labeling techniques have revolutionized the study of large proteins and protein complexes using biomolecular NMR spectroscopy. The methyl group is an ideal probe for investigating the structure, dynamics, and function of high molecular weight proteins, allowing for detailed analysis that would be challenging with conventional methods. At NMR-Bio, we are at the forefront of methyl labeling innovation, offering state-of-the-art solutions tailored to meet the diverse needs of protein research.
Our advanced methyl labeling techniques are essential for uncovering intricate protein behavior in both solution and solid-state environments. These isotopically enriched compounds are ideal for a broad range of NMR applications, enabling precise insights into protein folding, dynamics, and interactions. With the integration of deuterium and other labeling schemes (e.g. uniform 13C & 15N), NMR-Bio’s offerings provide unparalleled resolution for studying complex protein structures and functions at the molecular level.
NMR-Bio's flagship methyl labeling scheme: unlocking high-resolution insights with isotope-labeled amino acids
Methyl labeling can be achieved through either labeled amino acids or isotopically labeled precursors (such as keto acids). While labeled amino acids are highly applicable across various expression systems—prokaryotic, eukaryotic, and cell-free—certain labeled amino acids may be cost-prohibitive. This makes labeled precursors a more viable option, especially when working with E. coli culture. NMR-Bio specializes in the stereospecific labeling of leucine, valine, and regio-specific labeling of isoleucine, making it an ideal choice for high-precision NMR studies.
NMR-Bio’s methyl labeling kits for protein studies
NMR-Bio offers a range of user-friendly kits that simplify the protein labeling process, making it accessible for researchers at any stage of their study. Our kits are optimized for straightforward integration into E. coli culture medium before protein expression, ensuring seamless workflow integration. Each kit comes with detailed protocols for easy use, saving valuable time and resources.
Our kits facilitate the incorporation of 13CH3 or 13CHD2 isotopomers into methyl groups, and they also enable the creation of a linear 13C spin system connecting labeled methyl groups to the protein’s backbone nuclei, facilitating more accurate NMR assignments.
Depending on your specific needs, we offer the following kits:
Kits for 13CH3 Single and Combinatorial Methyl Groups Labeling
These kits allow you to label methyl groups in strategically chosen amino acids, enhancing the sensitivity and resolution of NMR data for high molecular weight proteins. Our solutions also reduce spectral complexity, enabling you to study challenging protein assemblies that traditional NMR methods cannot address. Additionally, these kits allow for the detection of long-range nOes and the extraction of precise long-range distance restraints between methyl probes in perdeuterated proteins. Compared to standard 2-keto acids, NMR-Bio acetolactate precursors increase sensitivity by up to 4x, enabling the detection of meaningful intermolecular restraints.
Kits for 13CHD2 Single and Combinatorial Methyl Groups Labeling
13CHD2 isotopomers are particularly advantageous for NMR dynamics studies, as they avoid complications related to dipolar cross-correlation that can arise with 13CH3 or 13CH2D. This isotopomer choice improves sensitivity and offers more accurate data for your analysis, especially in NMR experiments such as 13C relaxation studies.
Kits for 13CH3 or 13CHD2 Isotopomers with Linear 13C Spin Linking to Backbone
These advanced kits are specifically designed for Alanine, Isoleucine, Leucine, and Valine amino acids, enabling optimized labeling of side chains. The 13CH3 or 13CHD2 groups are connected to the 13N/13C backbone nuclei, providing unambiguous methyl group assignments for accurate NMR analysis.
Methyl labeling for advanced expression systems and cell culture.
For more complex expression systems, NMR-Bio’s methyl labeling strategies are versatile and can be applied to yeast, insect, mammalian cells, and cell-free protein synthesis. Our kits are particularly optimized for E. coli, but our custom synthesis options, including stereospecific valine labeling and regio-specific isoleucine labeling, allow for tailored solutions that meet your research’s specific demands.
Whether you’re exploring protein-protein interactions, protein structure, or the behavior of large protein complexes, NMR-Bio’s methyl labeling solutions provide precise, reliable tools for your studies. By selecting the right methyl groups and isotopic labels, you can gain deeper insights into protein function, dynamics, and structure, paving the way for more effective drug development and biomolecular research.