We are experts in protein engineering and isotopic labeling for precise NMR research.

At NMR-Bio, we understand that producing high-quality recombinant proteins is essential for structural biology, biopharmaceutical research, and drug discovery. Achieving optimal purity, proper folding, and functionality is critical to obtaining reliable results.

We enhance the value of these proteins through precise isotopic labeling, enabling advanced NMR studies for detailed structural analysis, protein dynamics, and protein-ligand interactions. From protein design to large-scale production, we optimize every step to ensure high yields, isotopic enrichment, and robust sample integrity.

Our expertise in uniform labeling, deuteration, and methyl-specific labeling delivers high-resolution data even for complex systems. Leveraging innovative protein engineering approaches, we design and produce tailored proteins that meet the exact requirements of your research.

We provide expert protein engineering and isotopic labeling services.

NMR-Bio offers a range of services designed to support advanced protein engineering, Our expertise spans the entire process, from gene design and mutagenesis to large-scale protein production and isotopic labeling for precise NMR analysis.

Gene design & Mutagenesis

We specialize in protein engineering, optimizing genes to enhance protein expression, stability, and solubility. Our approach includes:

Gene design and site-directed mutagenesis to introduce targeted modifications for specific research objectives.
Creating multiple constructs to identify the most suitable variant for high-yield production.
Codon optimization to maximize expression yields across various expression systems.

Feasibility study

Before proceeding with large-scale production, we conduct a thorough feasibility study to evaluate optimal conditions. This includes:

Developing expression and purification protocols
Assessing protein solubility, stability, and overall yield to establish scalable protocols.

Large-scale expression & Purification

Our facilities are equipped to handle large-scale protein production, ensuring high-quality samples for advanced research. We provide:

Scalable production using bacterial, insect, or mammalian systems.
Purification methods including Affinity, Ion Exchange, SEC, and other strategies tailored to the protein’s properties.

Isotopic labeling

We offer a comprehensive range of isotopic labeling services to enhance NMR analysis. This includes:

Uniform labeling with 13C, 15N, 2H for structural and dynamics studies.
Methyl-specific labeling for high-resolution data, especially for large protein systems.
Tailored isotopic enrichment strategies for advanced NMR analysis .

Quality control

Ensuring sample integrity is a priority. Our quality control process includes:

Assessment of purity, folding, and integrity using NMR, Mass Spectrometry (MS), and other biophysical techniques.
Providing stability data for complex molecules, enzymes, and engineered proteins.

Our customized protein production platforms for your research

At NMR-Bio, we offer tailored protein production platforms designed to support a wide range of biotechnological applications including protein engineering, structural biology, and NMR analysis. Our expertise encompasses multiple expression systems to accommodate proteins from various origins.

recombinant proteins where rapid synthesis and straightforward protein purification are essential.

residue modifications. This system supports a broad range of applications, from enzyme design to rational protein engineering.

and high-quality protein yields essential for accurate protein structural and binding studies.

of therapeutic targets like monoclonal antibodies. This system is particularly suited for studies involving protein domains, therapeutic applications, and engineered scaffold protein.

Our isotopic labeling services: precision for complex protein studies.

At NMR-Bio, we offer a comprehensive range of isotopic labeling services designed to enhance NMR studies of recombinant proteins, providing precise insights into protein structure, dynamics and interactions. Our tailored approaches include:

Uniform isotope labeling

An essential technique for standard structural and dynamics NMR studies, where all amino acids in the protein are uniformly labeled with one or a combination of isotopes such as 13C,15N and 2H.

Selective isotope labeling

We provide tailored labeling strategies targeting specific residues or regions of the protein to gain detailed insights into local structures, active sites, ligand interactions and catalytic substrates.

Deuteration & Methyl-specific labeling

Combining deuteration with methyl-specific labeling on key amino acids (Ile, Leu, Val, Met, Ala, Thr) improves NMR resolution, particularly for

high-molecular-weight proteins. This technique is crucial for advanced studies of complex machineries, enabling accurate analysis of protein dynamics, conformational changes, and protein-ligand binding.

Parallel Mutant Expression & Methyl Assignment

Through site-directed mutagenesis, we generate and purify multiple protein mutants in parallel. This strategy facilitates methyl group assignment in large proteins.

Why choose NMR-Bio for isotopic labeling?

Versatility

Our labeling techniques are applicable across various expression systems (bacterial, eukaryotic or cell-free), providing unmatched flexibility for protein synthesis and engineering.

Precision

We enhance NMR resolution and data quality through targeted isotopic enrichment, enabling accurate insights into biomedical applications and therapeutic protein design.

Expertise

With extensive experience in protein engineering, we optimize each labeling strategy to meet your specific research needs.

Through rigorous quality control processes, we guarantee sample integrity, empowering researchers to advance protein R&D, structural biology, and drug discovery. Whether you are working with enzymes, peptides, or complex molecular systems, NMR-Bio provides the expertise and technology needed to achieve your scientific goals.